Aminotransferase Alanine anda - uphawu izinqubo ukuvuvukala futhi esiwohlozayo kwezitho zomzimba

aminotransferase Ngenxa nokuthutha kusuka NH2-amaqembu nge γ-amino acid donor kuya acceptor - α-amino acid ngaphandle kumiswa Lesisemkhatsini ammonium. Okungukuthi, abangabantwana transamination (transamination: ukudluliswa esethola amaqembu acid ukuze keto acid) - inqubo ukuvulwa ososayensi adumile Russian futhi Kritsman Braunstein.

amino acid (aspartic, glutamic futhi alanine) ngesikhathi transamination ibe esihambelanayo α-keto acid, okuyizinto izingxenye tricarboxylic acid umjikelezo. Phakathi namachibi zabo emjikelezweni Krebs bangabantu umthombo wamandla.

Transamination ubalulekile ekunikezeni the urea umjikelezo aspartate.

Naphezu kweqiniso lokuthi kungenzeka ukusabela transamination wadalula kanye elimnandi amino acid: phenylalanine, tyrosine futhi tryptophan - ngokoqobo kwezokwelapha ithole incazelo umsebenzi aspartate futhi aminotransferase alanine. Lezi enzyme kuthiwa esakazwa emisipheni yomzimba womuntu.

aminotransferase Alanine anda amangqamuzana abomvu lokuhlushwa yayo liphindwe kasithupha ephakeme kuqhathaniswa ukuthi le serum igazi. Ubuningi esiphezulu enzyme ibizwa fixed emisipheni isibindi.

Alanine aminotransferase kuyinsakavukela akukhona ezicutshini zomzimba womuntu okufanayo. Ngokwesibonelo enzyme umsebenzi kwezicubu inhliziyo kuyinto 7.1 (E * 10-3 g izicubu homogenate), esibindini - 44, imisipha lwamathambo - 4,8, izinso - 19, ubende - 1.2, 0.7, kanye wamaphaphu serum - 0,016. izindlela ekhona ekutholweni umsebenzi aminotransferase e serum is zihlukaniswe ngamaqembu amabili: endpoint (colorimetric, usebenzisa ezivamile, hhayi ifakwe lokushisa elawulwa Cuvette photometric apharathasi) kanye kinetic (spectrophotometric).

B6 kuyinto Vitamin ezivamile nezinhlobonhlobo zokudla. Nokho, kufanele sikhumbule ukuthi ngesikhathi senqubo yokupheka (obrobka ezishisayo) anovithamini libhujiswa. Pyridoxine kuyinto cofactor e enzyme kakhulu. Lokuhlushwa pirodoksin-5-phosphate egazini kuyancipha njengoba ekhula. Ngakho-ke, umsebenzi waleli enzyme kuyinto alanine aminotransferase, kwehlile abantu abaneminyaka engama 64 (cishe 30%) kunalawo oneminyaka engu 46-63. I lokuhlushwa B6 egazini serum alanine aminotransferase izimbangela.

Ukuze sithole isimo sokusebenza kwesibindi ngokuvamile ihlolwe amazinga aminotransferase egazini lesiguli. Lokhu kungenxa ngokuyinhloko yokuthi izinguquko umsebenzi wabo kwenzeka ngokushesha kakhulu kunezinye izinto imingcele laboratory. Ukuqedwa (ephumayo) we enzyme isibindi egazini iyinkomba yokuthi cytolysis - cell ukubhujiswa noma ukuphazamiseka ulwelwesi yabo permeability. Lapho ehlaziya izinguquko umsebenzi enzyme ezibalulekile ukusungula indawo emzimbeni. Udinga ukwazi ukuthi umsebenzi wabo egazi kanye nomzimba onempilo, kwasendaweni subcellular futhi umzimba. Ubuningi enzyme - alanine lenyuka, ikakhulukazi emangqamuzaneni isibindi, ngakho-ke, ngisho ukulimala kancane kuphakamisa zabo ukwanda umsebenzi enzyme igazi. Banikezwe umsebenzi enzyme ukuxilonga izifo kwesibindi (i-hepatitis, steatosis).

aminotransferase Alanine anda necrosis kwesibindi, kanye parenchymal kwesibindi. Ubuningi eliphansi enzyme ibhalisiwe e-hepatitis chronic sokuwohloka. Kumele ukuthi ukwanda umsebenzi aminotransferase e serum iqala izinsuku 3-8 ngaphambi ukuqala izimpawu eziyinhloko basemtholampilo isifo futhi ifinyelela esiphezulu ezinsukwini zakuqala ukuthuthukiswa inqubo sokugembula.

Ngakho, aminotransferase alanine anda: isifo sokusha kwesibindi, jaundice, nomdlavuza wesibindi, isilethikusebenza, kwesokudla ukumelwa inhliziyo, hypoxia, ukushaqeka, myositis, myocarditis, steatosis, pancreatin, ukuba umlutha wotshwala ezingamahlalakhona.