Phosphatase Acid egazini: ukuzimisela, ekunika emagama izinga

Acid phosphatase --enzyme ngesivinini noqhekeko zishintshe emzimbeni. Ukunqunywa ezingeni layo egazini kubaluleke ekuxilongeni isimo sezempilo. Kunezinhlobo eziningana phosphatases acid, yabelana ezisebenza ezifanayo, kodwa ihluke nomunye maqondana ezicutshini zomzimba, umsuka chromosomal, okuqanjiwe.

Inani lamavoti

enzyme Powered e ezindaweni esidi, kungakho igama. phosphatase Acid itholakala kwezicubu ehlukahlukene amaseli kungenzeka ise lysosomes nangale. Inani elikhulu kunqunywe endlaleni engasesinyeni, kanye amaseli kwezitho ezilandelayo:

• isibindi;
• ubende;
• umnkantsha;
• egazi (erythrocyte, izinhlayiya zegazi macrophage).

Encwadini umzimba uphilile umsebenzi phosphatase acid kunalokho ongaphakeme. Laba bameleli be-lowesilisa isigamu ezingeni layo siqukethe phosphatase Prostatic, nokuthi lapho libhujiswa ngo amaseli isibindi igazi. I-enzyme of umzimba female kwakhiwa phansi ngokuphelele by sesibindi erythrocyte, nama-platelet.

Lysosomal phosphatase acid

It elise amangqamuzana amaningi. namaphutha alo kuyinto autosomal isifo esikhubazayo, okuyinto ubhekwa umphumela izinqubo sokugembula umzimba. Symptomatology lesi sifo kubonakala ngendlela elandelayo:

• isicanucanu ngakanye nokuhlanza;
• ubuthakathaka, ukulahlekelwa ukusebenza kahle;
• ukwehlisa umfutho wegazi;
• ebuntwaneni - opisthotonus (nokuquleka ukuthi zenzeka isiyaphuma we arc emuva, iziguli ancike kobuso embhedeni kuphela entanyeni izinyawo);
• ukopha.

Uma ezingeni enzyme alwanele kuphela amangqamuzana amhlophe egazi, iziguli bevame exacerbations izifo ezingamahlalakhona.

Prostatic acid phosphatase

Kuthathwa marker izinqubo umdlavuza wendlala. Lolu hlobo lwe-enzyme ibizwa kunqunywa futhi njengoba uketshezi imbewu umaka ebunzimeni (ukudlwengula). Acid phosphatase ophakamayo, futhi ngemva kwezinsuku ezimbalwa ukuhlinza indlala wendlala noma biopsy ngemuva.

Optimum pH inkomba ekusebenzeni kahle enzyme - 4.6. Umsebenzi ubonakala kuphela ngemva indoda ifinyelela ekuthombeni.

Okwamanje, sokunquma ezingeni phosphatase Prostatic njengendlela marker umdlavuza zivame ukufiphala ku isizinda nesithakazelo esikhulayo antigen wendlala eqondene, elikwaziyo ukuqinisekisa ukuba khona isifo emazingeni ngaphambili yentuthuko. Noma kunjalo, izinkomba Prostatic acid phosphatase kubalulekile ukubikezela ukuphinda inqubo umdlavuza ngemva kokuhlinzwa ukuze olukhulu wendlala ukususwa.

Uhlaziyo ukunquma ukuphumelela ukwelashwa of ingozi Hyperplasia Prostatic nezinye pathologies Prostatic.

eritrotsitarnaja phosphatase

Lena enzyme polymorphic ukuthi akuyona kuphela abomvu egazi ashaye kodwa futhi nezinye emangqamuzaneni omzimba. PH elikhulu ukusebenza kwawo kuhluka 5.2 kuya 6.2.

Esidi phosphatase igazi kungaba azimisele ukucacisa ubuyise. izinhlobo ezithile enzyme abahlangene izimo elandelayo:

• yofuzo ukuze anaemia hemolytic;
• zokugula, esibonakala by ukuthuthukiswa igazi hemolytic ngemuva kokudla imidumba;
• anaemia e ukusetshenziswa kwezidakamizwa ezithile noma ukuthuthukisa ukutheleleka ngegciwane imvelaphi ezibangelwa amagciwane kanye ezibangelwa amagciwane.

Kukhona izinhlobo erythrocyte acid phosphatase kuhlobane ngokuqondile yokuphula ezenzeka ebuntwaneni.

Macrophage uhlobo phosphatase acid

Enzyme kubalulekile sitholakele Gaucher isifo. Lena isifo ngofuzo esizibonakalisa sokuzuza kwesibindi, ubende, izinso, ubuchopho, iphaphu glucocerebroside (izinto eziphilayo ukusuka iqembu fat). Lesi sifo kuhambisana nokwanda okuphawulekayo ubukhulu yemizimba oshiwo ngenhla, ukwehla elibukhali, ngenani labo bonke egazi, ukuquleka, ukuquleka spastic, retardation engqondo.

Macrophage acid phosphatase Uphinde abhekwe umaka ethize enoboya cell wegazi. Lesi sifo sihambisana ukukhiqizwa amanani abalulekile enzyme, okuyinto kunqunywa egazini.

phosphatase osteoclasts

Ngokuthi osteoclasts ithambo amaseli obhekene ukubhujiswa kwalo. Lesi sinyathelo kubalulekile kakhulu amathambo ukukhula zilungiswe iyunithi. esinejubane kwamathambo esihambisana zifo ezilandelayo:

• Paget sika isifo (sokugembula zamagama evamile ithambo kwetinhlavu kanye izakhi zalo);
• hypercalcemia ka umdlavuza;
• hyperactivity we yegilo kanye parathyroid nezindlala ;
• postmenopause lihambisana kwamathambo.

Umphumela - waphuka njalo futhi thinning okuqhubekayo ithambo izakhiwo. Ukunqunywa acid phosphatase osteoclasts sikhombisa amazinga aphakeme enzyme isizinda pathologies ngenhla.

Ilungiselela nokuqhuba izifundo ukuhlola

izinkomba quantitative zinqunywa kwizimo zamalabhorekthi. Uhlaziyo kumele kwenziwe emva kwezinsuku 2 kusukela ngesikhathi ezinye izindlela zokuxilonga. Uma ufuna ukuhlola ezingeni Prostatic acid phosphatase, lokhu kufanele kwenziwe ngaphambili kuka 48 amahora emva biopsy, palpation we wendlala yabesilisa, isinye catheterization. Endabeni okuphambene, imiphumela ingase ibe positives bamanga.

Fence impahla ucwaningo kwenziwe esiswini esingenalutho. Ungakwazi uphuze amanzi kuphela, aphuza itiye, ikhofi, ayo nezinye izinhlobo zophuzo akuvunyelwe. Emini, udinga ukuba bangayeki utshwala, okunamafutha, izitsha ngibhema 1 ihora ngaphambi ukuhlaziya - ubhema. I last yehora ngaphambi edonsa impahla kumele iqhutshwe nokuzola umnyuziki ngokomzwelo.

Ukuze sitholakele igazi emithambo yegazi isetshenziswa, okungukuthi serum yayo ukugwema hemolysis. Imiphumela inhlolovo zaziwa ngosuku olulandelayo ngemva kwesifundo.

Indlela esetshenziswa

Ukuze sicacise ezingeni Imininingwane acid phosphatase esetshenziswa amasu immunochemical nge ukuthola chemiluminescent. Sebenzisa substrates ekhethekile uxhumana enzyme ethile. Okwamanje izinhlelo eziwasebenzisayo luminol nemikhiqizo peroxidase lihambisana isixazululo i-hydrogen peroxide. Lo mphumela sengezwe umqalisi (isib n-yodofenol) uyakwazi okwandisa luminescence izixazululo izikhathi latinkhulungwane letinyentana.

Zikhona nezinye izinhlelo ezisebenzisa ane-alikhali phosphatase futhi AMPPD substrate, okwenza indlela zokuxilonga ebucayi nakakhulu. Izinzuzo indlela kukhona ukufunda ukuzinza reagents esetshenziswa, akukho ingozi umonakalo omkhulu obangelwa imisebe. Ukulinganiselwa esekelwe yinkimbinkimbi inqubo yokuhlola.

kuyinsakavukela

Ubude acid phosphatase assays wabonisa U / L. nemingcele yayo ovumelekile iyashiyana yobudala nobulili.

Jwayelekile Prostatic acid phosphatase izinkomba - esingaphansi 2.1 ng / ml. Wonke la manani ezingenhla zibonisa umphumela omuhle uphethwe.

Uma izinga le-enzyme anda?

Imibandela sina kakhulu lapho izinyathelo ambalwa ngaphezu evamile:

• thrombocytopenia - inombolo ukuncipha egazi obhekene ekunqandeni yayo;
• thromboembolism - ukuvaleka inesikhala main Umthambo thrombus, bathinteka odongeni isitsha, noma embolus, enisezweni egazini;
• isifo Hemolytic - isifo ephelezelwa nomonakalo omkhulu kwamangqamuzana egazi abomvu ukukhulula amanani abalulekile bilirubin esegazini;
• okuqhubekayo Paget sika isifo - amathambo zokugula apharathasi lapho Ukungadli phakathi ithambo kwetinhlavu kanye ukubhujiswa izingxenye esivuna yokugcina;
• myeloma - abulalayo inqubo isimila imvelo, okuyinto ubuhanjelwe B-lymphocyte, obhekene synthesis amaseli zokuzivikela;
• Niemann Khetha isifo - zokugula lezinceku libhekene kahle amafutha umzimba ekuqongeleleni lipids kwezitho ezinkulu (isibindi, ubende, kwamaphaphu, inhliziyo, izinso);
• izinsuku ezimbalwa emva kokuhlinzwa phezu endlaleni engasesinyeni nezicubu yayo biopsy.

Ene-asidi, phosphatase ane-alikhali - iqembu enzyme obhekene izinqubo ezibalulekile emzimbeni. Kuyinto ngosizo lwabo zivela esibalulekile ukusabela elandelanayo. Icacisa ezingeni enzyme ivumela isikhathi uhlole ubukhona zokugula futhi ngiwuphatha ukusekela ukulungiswa okwengeziwe izinga okusezingeni zokuphila.