Culturing engapheli eyayiqhutshwa zemibungu ngaphandle kwesibeletho. Ukutshalwa gametes futhi imibungu - kuyini?

Sekuyiminyaka engaphezu kwengu-amabili e imibhangqwana unezinkinga akhulelwe azale ingane, sathola ithuba ngesimo ngaphandle kwesibeletho Uhlelo kokukhulelwa. Yiqiniso, okwamanje kungokuhluke inqubo eqolo, umphumela okungadingi njalo zakwazi ukwenza lokho okulindelwe iziguli, kodwa namanje ithuba kulabo abafuna ingane yabo zofuzo. umbungu isiko ivumela ukukhetha ezinye izifo zofuzo kuphela iphila kunazo, kanye ukuqeda.

amagama

Ngo kokukhulelwa kwesibeletho - kuyinto isiqondiso ubuchwepheshe zokuzala, okubandakanya ukuzalanisa iqanda ngaphandle wesizalo sikamama begodu i-embryo podsazhivaniya isilungele izinsuku ezinhlanu nesikhombisa.

Ukutshalwa zemibungu - ukusekela ukuthuthukiswa futhi ukuphila imibungu phambi iphinde itshalwe nabo kwesibeletho.

izigaba IVF

inqubo-IVF siqukethe izinyathelo eziningana noma izigaba:
1. Ukugqugquzela ka superovulation, parallel ukuqapha follicle genesis futhi ekukhuleni endometrium.
2. wokubhoboza follicle.
3. oocyte kokukhulelwa ngaphandle kwesibeletho kanye imibungu culturing.
4. Artificial silondoloze esigabeni luteal we umjikelezo wokuya esikhathini.
5. umbungu ngokuxhunyelelwa futhi uphethwe yokukhulelwa.

Kwabesifazane abasebasha ithuba IVF ngaphandle superovulation.

Izinkomba kanye Izimo for yokusizwa

Isincomo salokhu zokukhwabanisa sezokwelashwa khona ukungazali lowesifazane olumelana yokwelapha kodokotela, inqobo IVF inikeza amathuba eliphezulu kakhulu ukukhulelwa.

Izimo ziyefana:
- extragenital zokugula, okusongela isisu;
- kombungu futhi deformations kwesibeletho;
- izimila esibelethweni futhi ezikhiqiza amaqanda;
- izifo sibi ukuvuvukala (hhayi kuphela uhlelo zokuzala);
- khona umdlavuza emlandweni.

Ukulungiselela i-IVF

Isigaba sokuqala - ukuqoqwa ukuhlaziywa kanye ukuthola pathologies. Ukuze wenze lokhu, isiguli kumele kuhlolwe igazi-hormone, kanye cwaningo ezinemininingwane oyisazi samakhemikhali ezinto eziphilayo jikelele kanye tollerantnost glucose. Okwenziwe ngokuzimisela efanele ku izifo urogenital kanye ultrasound Izitho zokuzala yangaphakathi.

Umlingane Osebenzelana Naye uzakwenzela ukuhlaziywa isidoda ukuqinisekisa ukungabi ukungabi nenzalo wesilisa. Ngempela ukuthatha smear Cytology futhi colposcopy ukubekela eceleni isimo pre-anomdlavuza. umama okhulelwe aba siqhuba isifundo ku Torch izifo (rubella, toxoplasmosis, i-herpes, cytomegalovirus futhi chlamydia), njengoba lokhu kungase kuthinte ukuthuthukiswa esizayo umbungu. Ekupheleni kwe-ultrasound scan we nezindlala mammary futhi yegilo.

Isinyathelo sesibili Kwenziwa ngokushesha ngaphambi ukwelashwa. Siqukethe amaphuzu amathathu:
- abashadile kumelwe iphumelele ekuhlolweni i-HIV, i-hepatitis viral, ugcunsula, uthole imiphumela eqenjini labo igazi RH isici;
- Abesifazane kufanele uhlolelwe i-ushukela egazini, ephelele kakhulu segazi, umchamo, oyisazi samakhemikhali ezinto eziphilayo, kanye ukwazi nesikhathi coagulation;
- umama okhulelwe kumele baye baphetha lo owelapha ukuthi azikho contraindications sokukhulelwa.

Inqubo ye-IVF

Okokuqala udokotela, ngemva kwalo lonke uvivinyo ezidingekayo, uqhuba ukushukuma wesibeletho owesifazane ukuze uthole amaqanda enempilo. Ukwethulwa izidakamizwa kuthoma ngesikhathi usuku 21 umjikelezo wokuya esikhathini, futhi ihlala isikhathi esingangenyanga. Phakathi nalesi sikhathi, udokotela ngokuqhubekayo uqaphe, njengoba follicle ukuvuthwa futhi eyakha endometrium esibelethweni usebenzisa ultrasound. Futhi njengoba owesifazane uthethe ihlaziya hormone, ukuze ukukubona konke okuhilelekile. Njengoba nje iqanda ripens, inqubo sinenkonzo yokunakekela iziguli emakhaya eyenziwa ehoxile. Nokho, le nqubo kungavimbela ezinye zezici:
- ukuwaphendula wesibeletho stimulation;
- khona ukuvuvukala kule ingaphakathi okhalo;
- ukukhiqizwa kwamaqanda ngaphambi kwesikhathi;
- anovulatory umjikelezo (lapho follicle ovuthiwe aqukethe iqanda). Zonke lezi zici kufanele kubhekwe ngaphambi ezincoma umbhangqwana ukuqala IVF.

Njengoba nje ubaba esizayo noma ukunikela ngezitho engaziwa kuzodlula impahla yabo zofuzo kungaba kwenziwe inqubo kokukhulelwa. eside isiko kwesibeletho zemibungu kuyadingeka ukuze ukhethe imibungu iphila kunazo. Emva kwezinsuku eziyisihlanu noma eziyisithupha emva kokukhulelwa, imibungu eziningana zidluliselwa esibelethweni esibelethweni sikamama. Ngemva kwamasonto amabili, umama okhulelwe kaButhelezi igazi ezingeni human chorionic gonadotropin (hCG) ukunquma sokukhulelwa.

ukutshalwa engapheli eyayiqhutshwa zemibungu

Ngokuvamile, uma kuhlinzwa iziguli ezifuna ziphathwa bathole usizo lwezempilo eqondile, kunemibuzo eminingi. Omunye wabo uya like lokhu: "Usiko gametes futhi imibungu, kuyini na?", Impendulo ulula futhi eziyinkimbinkimbi ngesikhathi esifanayo. Lena indlela lapho umbungu uyaphila ukuze zako emzimbeni kamama. ukutshalwa engapheli eyayiqhutshwa zemibungu ngaphandle kwesibeletho inqubo eqolo, zobuchwepheshe ukuthi kudinga ilwazi elithile namakghono. laboratories Modern njalo ngcono inqubo sokukhulelwa kanye nemishini. I emisha zakamuva - ukutshala imibungu EmbrioGen imvelo. Engeziwe nezici ukukhula ahlinzeke awokuthi umbungu selitshalwa odongeni lesizalo.

Ngaphambili, ukutshala imibungu wanika avuke kuphela esigabeni ichoboza futhi afakwe kwesibeletho has izinsuku ezintathu emuva kwalesi "ebamba isisu", kodwa bayoba nezimpande ngaphansi kuphela ingxenye yesine yayo yonke amacala IVF. Kodwa ngemva ekusunguleni indlela ngaphandle kwesibeletho isiko zemibungu esigabeni blastocyst, ukuphumelela indlela liye lenyuka. Lokhu kwakudinga entsha namasiko abezindaba kanye netindzawo.

Izinzuzo nezingozi nebubi inqubo

inqubo enjalo ukubonisa isiko zemibungu ezingeni elilandelayo.

1. Kuyasiza ukhethe ethembisayo ngokuya yenzeka imibungu.
2. It kunciphisa inani chromosomal kwezinso, njengoba esigabeni blastocyst kungenzeka ukulandelela futhi kuvimbele.
3. It has indlela bokuphila ngaphezulu.
4. It kunciphisa ingozi kaningana, ngoba sekwanele ezimbili nje imibungu esikhundleni ezinhlanu, njengasekuqaleni, ukukhulelwa.
5. ukhulela ethunjini kwenzeka ngezikhathi esingaphansi ngaphambili.

Nokho, kukhona nemibi le nqubo. Esokuqala siwukuthi angakhula akubona bonke nuclei esigabeni oyifunayo. Namanje incubator uhlelo namasiko abezindaba usadinga ngcono, eduze ngangokunokwenzeka ukuze izimo zemvelo. Uma imibungu engaphansi kwemine uyasinda ke ematfuba ukuthi ngeke balungele iphinde itshalwe, incane kakhulu. Enye lokususa, njengasezulwini izinguqulo ezandulele IVF - asikho isiqinisekiso ngokuphelele impumelelo. Ayikho uhlelo udokotela-zokuzala emhlabeni ngeke ukuth bathi le ndlela iqiniso eliphelele. Ososayensi kusadingeka balwele ukuzifinyelela.

imiphumela emibi-IVF

imiphumela emibi ngemva IVF, ngesikhathi, esetshenziswa isikhathi eside zemibungu yilezi:
- nokukhulelwa izingane eziningi ngasikhathi imibungu njengoba amaningi adluliselwe esibelethweni;
- ukhulela ethunjini (ukunyakaza ayikwazi ayifakiwe umbungu ngaphakathi kwesibeletho);
- ukushukuma ngokweqile ezikhiqiza amaqanda (wesibeletho hyperstimulation syndrome kuholela nenzalo);
- ukutheleleka ukopha.