Lokhu kwakwenziwa ngayiphi injongo isampuli labetibambile Coombs?

Coombs - ukuhlolwa ethize laboratory evumela ukucacisa masosha omzimba plasma noma abomvu egazi ebusweni. Le nqubo ivumela ukuxilonga mzimba anaemia hemolytic, kuhlanganise kuzalwa, kanye ukuhlonza hemolytic ukumpontshelwa ukusabela. Coombs ngenkuthalo lisetshenziswe isayensi forensic nofuzo ucwaningo ukucacisa antigen erythrocyte. Ukuhambisana zonke imithetho azihlole kwenza kube nokwenzeka ukuba uthole imiphumela enokwethenjelwa kakhulu.

Injongo ukuhlolwa antiglobulin

Direct Coombs test kungathola amasosha omzimba elwisana erythrocytic, okuyinto asethe njó erythrocyte. ukuphendula Positive lapho socwaningo olufanayo ukhomba ukuthuthukiswa sokuhlasela kwamasosha omzimba anaemia hemolytic. Kufanele kuqashelwe ukuthi umphumela othi awunalo igciwane akusho ukubeka eceleni khona isifo sokuhlasela kwamasosha omzimba, ngoba amasosha omzimba ngokuvamile atholakala ifomu mahhala, isb azihlangene erythrocyte. Ezimweni ezinjalo kuhle ukuba sifeze engaqondile Coombs test, okuzokwenza ukucacisa ejenti ezizimele e serum.

iyona ukuhlaziywa kanjani?

W Kuyinqwaba igazi emithambo yegazi isiguli wenziwa esiswini esingenalutho ekuseni, naphezu kweqiniso lokuthi noma yimaphi zici eziphawulekayo enomthelela yi sokugcina salolu test, ayivezi. Isitolo impahla kuvunyelwe ezithathwe ° 2 kuya ku-8 C hhayi isikhathi eside kunaleso izinsuku eziyisikhombisa. Ukuze izinkomba yalolu cwaningo babenjengomuntu eqondile ngangokunokwenzeka, lonke igazi phakathi yokuqala amahora amabili ukuba anikezwe i-laboratory. Kungakuhle Coombs isampula kumele sibonise yi negative, okubonisa ukungabi khona izinguquko hemolytic emzimbeni.

sokuqonda Inani lamavoti

Coombs kunalokho kukhathaza ucwaningo indlela edinga ngokucophelela futhi enembile. Lapho usebenzisa i-kungase novivinyo olunjalo engaqhamuka obuthile ukuthi abahlangene incazelo engalungile imiphumela yokugcina ngenxa ukubonakaliswa ezibuthakathaka basabele kahle. Kufanele kuqashelwe ukuthi ukuhlaziywa kungathembeki - okungukuthi Coombs omuhle - kungaba umphumela iyasilela egeza erythrocyte contact nge okunamafutha
surface futhi neutralization izingxenye antiglobulin reagents serum. Enye Ububi le ndlela kuyinto nokuntengantenga ka efundwayo kuthathwe imali okuyinto inezici ezithile.

Imbangela yi zamanga-emibi kungaba ngokweqile shaking ekumisweni kwe erythrocyte e resuspension. Imiphumela yokuphambuka kungase futhi kube ngenxa yokubakhona anticomplement ukungcola omzimba zazo adsorbed phezu ngesikhathi bayashintshana ekufukameleni we erythrocyte test, ngaleyo ndlela kwakhele ukubukeka Chile oqondile. Uma ugeza ngokucophelela amasampula yokuhlola nokuqapha izimo ukuphendula, lezi yokuntula kalula luqedwe, okuzokwenza ukwandisa amathuba okuthola izinkomba onokwethenjelwa kunayo yonke Coombs test.